Speaker: Dr. Chang Lu, Associate Professor of Chemical Engineering at Virginia Tech
Date: Wednesday, April 18, 2012
Time: 2:45-3:45 pm
Location: 310 ICTAS, 325 Stanger St.
Microfluidics provides a versatile platform for manipulating and analyzing cells down to single cell level. In this talk, I will discuss microfluidic devices we developed for genetic modification and cellular analysis. We developed a simple microfluidic flow-through technique that conducts electroporation under constant voltage for gene delivery. We showed that hydrodynamics such as Dean flows could be introduced to dramatically influence gene uptake and its distribution on the cell surface. We also developed a set of microfluidic cytometric tools for studying the subcellular localization of proteins and the cell surface events with high throughput and single cell resolution. Finally, I will briefly discuss our recent work on analyzing epigenetics of cells using chromatin immunoprecipitation assay. These tools may find applications to cell-based therapies, molecular biology studies, drug discovery, and clinical diagnosis/staging.